PLSCR3 Knockout cell line(3D4/21)

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location： Home > Products > KO Cell Lines > PLSCR3 Knockout cell line(3D4/21)

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PLSCR3 Knockout cell line(3D4/21) Order

PLSCR3 Knockout cell line(3D4/21)
Catalog#
YKO-R008
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PLSCR3 Knockout cell line(3D4/21)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PLSCR3 Knockout cell line (3D4/21)
Morphology
Macrophage-like, adherent
Passage ratio
1:2~1:3
Species
Pig
Gene Name
PLSCR3
Gene ID
102163020
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose
Target region
Knockout region: Exon 3 (based on transcript PLSCR3-201)
Validation: PCR+Sanger Sequencing
PLSCR3 Knockout cell line(3D4/21) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PLSCR3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 3D4/21 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PLSCR3 gene knockout PLSCR3 Knockout cell line (3D4/21) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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